School of Biosciences

Join us in our journey of discovery

Protein Form and Function Group

The biological properties of a protein depend critically on its 3-dimensional shape and hence proteins need to fold to reach their functional states. Our work is currently focused on a catalyst of protein folding – protein disulphide isomerase. This protein accelerates the rate of disulphide bond reduction and formation enabling the substrate to reach its folded structure more quickly. Our group is using NMR and other biophysical techniques to characterize this process in more detail at a molecular level. NMR spectroscopy is a technique used to study the structure, binding interactions and the dynamic nature of biological molecules including cellular metabolites, metabolic pathway intermediates, peptides and proteins. Our expertise includes the design and implementation of novel NMR experiments as well routine methods to solve biological problems.  NMR based projects aim to understand the relationship of structure to function in biomedical or biochemical systems and our project base includes protein folding and cancer cell molecular recognition.

Research within the NMR facility covers many areas and disciplines across the School of Biosciences and through external collaborations. These include:

  • structural characterisation of biochemically and biomedically relevant biomolecules
  • investigation of the dynamic properties of proteins and peptides using NMR relaxation
  • characterisation of protein-ligand interactions
  • high resolution spectroscopy of intermediates from biosynthetic pathways
  • identification of components in crude biological samples
  • design of novel NMR and structural methodologies
  • applied computational methods and software development for both NMR and structural biology

Group members:
Professor David Brown, Professor Mike Geeves, Dr Mark Howard, Dr Peter Klappa, Dr Richard Williamson, Dr Wei Feng Xue.

  • immunocytochemistry on mammalian cells
    immunocytochemistry on mammalian cells
    immunocytochemistry on mammalian cells

Enquiries: Phone: +44 (0)1227 823743

School of Biosciences, University of Kent, Canterbury, Kent, CT2 7NJ

Last Updated: 07/10/2013