The Moore Lab - Synthetic Biology for Natural Products For full details of the group, please visit the official website or follow on twitter. www.simonmoorelab.com https://twitter.com/sjmoore505
Simon re-joined the School in September 2018 as a Lecturer in Molecular Biology. He previously studied for a PhD in Biochemistry at the University of Kent with Professor Martin Warren. A Postdoctoral position followed, with Professor Paul Freemont and Dr Karen Polizzi at Imperial College, London.
ORCID ID: 0000-0002-1968-206X
Synthetic Biology for Natural Products
Natural products from soil bacteria Synthetic Biology
Cell-free protein synthesis
MSc-R projects available for 2021
Mining for antimicrobial drugs with synthetic biology
Synthetic biology is accelerating the discovery of promising new antibiotics and anti-tumour drugs, from microbial genomes. This will project will use Gibson DNA assembly to assembly so-called “cryptic” biosynthetic gene clusters encoded from microbial genomes. We will screen for antimicrobial activity, characterise for cluster function (RT-PCR, SDS-PAGE) and perform natural product purification (e.g. chromatography, HPLC, NMR).
Streptomyces Cell-free Protein Synthesis
Jointly supervised with Tobias von der Haar
Cell-free protein synthesis is a rapidly developing area in synthetic biology for the implementation of the design-built-test-learn cycle. In this project, we are interested in the potential of a Streptomyces cell-free system for studying gene expression tools and the synthesis of novel antimicrobials.
This project will involve Golden Gate DNA assembly and routine techniques in gene expression analysis.
Cell-Free Synthetic Biology: https://www.frontiersin.org/research-topics/12103/cell-free-synthetic-biology
Streptomyces CFPS: https://www.biorxiv.org/content/10.1101/2020.11.16.384693v2.full
An automated platform for ESKAPE pathogen antimicrobial discovery
The ESKAPE pathogens (Enterococcus faecium, Staphylococcus aureus, Klebsiella pneumoniae, Acinetobacter baumannii, Pseudomonas aeruginosa and Enterobacter spp) are multidrug-resistant bacteria found in nosocomial clinical infections and a growing concern for hospitals; novel antibiotics with distinct modes of action are required. We are interested in the development of an automated workflow for novel antimicrobial discovery. We will specifically study methicillin-resistant Staphylococcus aureus (MRSA) for drug screening using a unique approach to detect bioactivity, potentially at a high-throughput scale.
PhD student and Research Master applications from UK, EU, & Overseas are always considered. Various funding sources and scholarships can be explored for PhD studentship applications. Please send your CV and summary of your research interests to: firstname.lastname@example.org